Comparative Analysis of Antioxidant Properties of Cold and Hot Water Leaf Extract of Rauvolfia vomitoria Afzel
DOI:
https://doi.org/10.22452/mjs.vol45no1.4Keywords:
Rauvolfia vomitoria, antioxidant, free radicals, inhibition concentration, extractAbstract
Rauvolfia vomitoria Afzel has attracted significant research interest due to its multiple health benefits. Its antioxidant properties are important in preventing oxidative stress, cancer, heart problems, and neurological disorders. This study compares the antioxidant capacity of R. vomitoria leaf extract in both hot and cold water. Standard methods were used to prepare leaf extracts in both hot and cold water, which were subjected to different antioxidant assays. The hot water leaf extract had a better capacity to snare free radicals produced by stable DPPH's free radical at 50% inhibition concentration (3.07 ± 0.54 mg/ml) than the cold water leaf extract (9.82 ± 0.01mg/ml). The nitric oxide inhibitory capacity of R. vomitoria at 50% inhibition concentration in cold and hot water leaf extract (3.64 ± 0.38 and 4.24 ± 0.11) showed no significant difference. The hot water leaf extract had higher ferric-reducing antioxidant power (IC50 of 7.21 ± 0.07 mg/ml) than the cold water extract (10.85 ± 0.50 mg/ml). The hot water leaf extract showed a higher total antioxidant capacity (IC50 of 6.01 ± 0.21 mg/ml) than the cold water extract (9.01 ± 0.07 mg/ml). These antioxidant evaluations show that R. vomitoria hot water leaf extracts were superior to cold water leaf extracts as antioxidants and free radical scavengers. The findings show that R. vomitoria's hot-water extract has greater antioxidant activity than its cold-water extract.
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